Journal: STAR Protocols

Article Title: Protocol for investigating tertiary lymphoid structures in human and murine fixed tissue sections using Opal™-TSA multiplex immunohistochemistry

doi: 10.1016/j.xpro.2022.101961

Figure Lengend Snippet: Detailed staining conditions and order of the Opal™-TSA mIHC 7-marker panel for human FFPE tissue sections

Article Snippet: Rabbit polyclonal anti-mouse CD19 antibody , Cell Signaling Technology , Cat#3574 (1:200 dilution).

Techniques: Staining, Concentration Assay, Incubation

Journal: STAR Protocols

Article Title: Protocol for investigating tertiary lymphoid structures in human and murine fixed tissue sections using Opal™-TSA multiplex immunohistochemistry

doi: 10.1016/j.xpro.2022.101961

Figure Lengend Snippet: Detailed staining conditions and order of the Opal™-TSA mIHC 6-marker panel for formalin free zinc salt-fixed tissue sections

Article Snippet: Rabbit polyclonal anti-mouse CD19 antibody , Cell Signaling Technology , Cat#3574 (1:200 dilution).

Techniques: Staining, Incubation

Journal: STAR Protocols

Article Title: Protocol for investigating tertiary lymphoid structures in human and murine fixed tissue sections using Opal™-TSA multiplex immunohistochemistry

doi: 10.1016/j.xpro.2022.101961

Figure Lengend Snippet: Multiplex manual staining of a representative human lymph node FFPE tissue section using the 7-marker TLS panel Representative region of interest (ROI) showing merge (on left) and single staining (on right): CD4 (red), CD8 (magenta), CD19 (green), CD21 (yellow), DC-LAMP (orange), PNAd (cyan), and DAPI (blue). Images were taken at 20× magnification, and scale bars indicate 100 μm.

Article Snippet: Rabbit polyclonal anti-mouse CD19 antibody , Cell Signaling Technology , Cat#3574 (1:200 dilution).

Techniques: Multiplex Assay, Staining, Marker

Journal: STAR Protocols

Article Title: Protocol for investigating tertiary lymphoid structures in human and murine fixed tissue sections using Opal™-TSA multiplex immunohistochemistry

doi: 10.1016/j.xpro.2022.101961

Figure Lengend Snippet: Multiplex automated staining of a representative human lymph node FFPE tissue section using the 7-marker TLS panel Representative region of interest (ROI) showing merge (on left) and single staining (on right): CD4 (red), CD8 (magenta), CD19 (green), CD21 (yellow), DC-LAMP (orange), PNAd (cyan), and DAPI (blue). Images were taken at 20× magnification, and scale bars indicate 100 μm.

Article Snippet: Rabbit polyclonal anti-mouse CD19 antibody , Cell Signaling Technology , Cat#3574 (1:200 dilution).

Techniques: Multiplex Assay, Staining, Marker

Journal: STAR Protocols

Article Title: Protocol for investigating tertiary lymphoid structures in human and murine fixed tissue sections using Opal™-TSA multiplex immunohistochemistry

doi: 10.1016/j.xpro.2022.101961

Figure Lengend Snippet: Proposed grading system for the accurate classification of immune cell infiltrates, immune cell aggregates, immature TLSs and mature TLSs using the 7-marker TLS panel on human prostate cancer FFPE tissue sections Representative ROIs showing merge staining: CD4 (red), CD8 (magenta), CD19 (green), CD21 (yellow), DC-LAMP (orange), PNAd (cyan), and DAPI (blue). Images were taken at 20× magnification, and scale bars indicate 100 μm.

Article Snippet: Rabbit polyclonal anti-mouse CD19 antibody , Cell Signaling Technology , Cat#3574 (1:200 dilution).

Techniques: Marker, Staining

Journal: STAR Protocols

Article Title: Protocol for investigating tertiary lymphoid structures in human and murine fixed tissue sections using Opal™-TSA multiplex immunohistochemistry

doi: 10.1016/j.xpro.2022.101961

Figure Lengend Snippet: Multiplex manual staining of a Grade 2 immature TLS (upper panel) and a Grade 3 mature TLS (lower panel) in human melanoma tissue sections using the 7-marker TLS panel Representative ROIs showing merge (on left) and single staining (on right): CD4 (red), CD8 (magenta), CD19 (green), CD21 (yellow), DC-LAMP (orange), PNAd (cyan), and DAPI (blue). Images were taken at 20× magnification, and scale bars indicate 100 μm.

Article Snippet: Rabbit polyclonal anti-mouse CD19 antibody , Cell Signaling Technology , Cat#3574 (1:200 dilution).

Techniques: Multiplex Assay, Staining, Marker

Journal: STAR Protocols

Article Title: Protocol for investigating tertiary lymphoid structures in human and murine fixed tissue sections using Opal™-TSA multiplex immunohistochemistry

doi: 10.1016/j.xpro.2022.101961

Figure Lengend Snippet: Multiplex automated staining of a Grade 2 immature TLS (upper panel) and a Grade 3 mature TLS (lower panel) in human prostate cancer tissue sections using the 7-marker TLS panel Representative ROIs showing merge (on left) and single staining (on right): CD4 (red), CD8 (magenta), CD19 (green), CD21 (yellow), DC-LAMP (orange), PNAd (cyan), and DAPI (blue). Images were taken at 20× magnification, and scale bars indicate 100 μm.

Article Snippet: Rabbit polyclonal anti-mouse CD19 antibody , Cell Signaling Technology , Cat#3574 (1:200 dilution).

Techniques: Multiplex Assay, Staining, Marker

Journal: STAR Protocols

Article Title: Protocol for investigating tertiary lymphoid structures in human and murine fixed tissue sections using Opal™-TSA multiplex immunohistochemistry

doi: 10.1016/j.xpro.2022.101961

Figure Lengend Snippet: Proposed grading system for the accurate classification of immune cell infiltrates, immune cell aggregates, immature TLSs and mature TLSs using the 6-marker TLS panel on murine gastric cancer tissue sections Representative ROIs showing merge staining: CD4 (red), CD8 (magenta), CD19 (green), CD21 (yellow), PNAd (cyan), and DAPI (blue). Images were taken at 20× magnification, and scale bars indicate 100 μm.

Article Snippet: Rabbit polyclonal anti-mouse CD19 antibody , Cell Signaling Technology , Cat#3574 (1:200 dilution).

Techniques: Marker, Staining

Journal: STAR Protocols

Article Title: Protocol for investigating tertiary lymphoid structures in human and murine fixed tissue sections using Opal™-TSA multiplex immunohistochemistry

doi: 10.1016/j.xpro.2022.101961

Figure Lengend Snippet: Multiplex manual staining of a Grade 2 immature TLS (upper panel) and a Grade 3 mature TLS (lower panel) in murine gastric cancer tissue sections using the 6-marker TLS panel Representative ROIs showing merge (on left) and single staining (on right): CD4 (red), CD8 (magenta), CD19 (green), CD21 (yellow), PNAd (cyan), and DAPI (blue). Images were taken at a 20× magnification, and scale bars indicate 100 μm.

Article Snippet: Rabbit polyclonal anti-mouse CD19 antibody , Cell Signaling Technology , Cat#3574 (1:200 dilution).

Techniques: Multiplex Assay, Staining, Marker

Journal: STAR Protocols

Article Title: Protocol for investigating tertiary lymphoid structures in human and murine fixed tissue sections using Opal™-TSA multiplex immunohistochemistry

doi: 10.1016/j.xpro.2022.101961

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-mouse CD19 antibody , Cell Signaling Technology , Cat#3574 (1:200 dilution).

Techniques: Polymer, Plasmid Preparation, Recombinant, Saline, Software, Staining, Imaging

Journal: Frontiers in Veterinary Science

Article Title: Integrating miRNA and mRNA Profiling to Assess the Potential miRNA–mRNA Modules Linked With Testicular Immune Homeostasis in Sheep

doi: 10.3389/fvets.2021.647153

Figure Lengend Snippet: Integrated miRNA-target negative correlation regulatory networks. (A) Immune-related gene CD19 was potentially regulated by 24 known miRNAs. (B) Three genes related to the blood–testis barrier function, namely, CLDN11, ITGA6, and ITGB1, were potentially regulated by 87 known miRNAs. (C) Four genes that have been reported as testicular immune homeostasis-related genes, including TGFB1, TGFBR2, TGFBR3, and FASLG, were potentially regulated by 117 known miRNAs. (D) Fifty-one DE genes were potentially regulated by miR-146-5p, an miRNA related to immune homeostasis maintenance. The circle nodes represent miRNAs; the triangle nodes suggest target genes. The differentially expressed miRNAs/target genes are highlighted in red and blue, describing higher and lower expression in the T1Y group compared with the T3M group, respectively. T3M, testes from 3-month-old sheep; T1Y, testes from 1-year-old sheep.

Article Snippet: Membranes were incubated with rabbit polyclonal anti-CD19 antibody (Bioss, Beijing, China; 1:500 dilution), rabbit polyclonal anti-TGF beta receptor II antibody (Bioss, Beijing, China; 1:500 dilution), or rabbit polyclonal anti-beta-actin antibody (Bioss, Beijing, China; 1:1,500 dilution) and the secondary antibody goat anti-rabbit IgG conjugated with HRP (Bioss, Beijing, China; 1:5,000 dilution).

Techniques: Expressing

Journal: Frontiers in Veterinary Science

Article Title: Integrating miRNA and mRNA Profiling to Assess the Potential miRNA–mRNA Modules Linked With Testicular Immune Homeostasis in Sheep

doi: 10.3389/fvets.2021.647153

Figure Lengend Snippet: Expression and localization patterns of CD19 and TGFBR2 proteins. (A) Western blot analysis. (B) Relative protein expression. Data represent the mean ± SD. (C) Immunofluorescence staining. Blue, DAPI; red/CY3, CD19; green/FITC, TGFBR2. Scale bar, 50 μm. T3M, testes from 3-month-old sheep; T1Y, testes from 1-year-old sheep; DE, differentially expressed. ** P < 0.01.

Article Snippet: Membranes were incubated with rabbit polyclonal anti-CD19 antibody (Bioss, Beijing, China; 1:500 dilution), rabbit polyclonal anti-TGF beta receptor II antibody (Bioss, Beijing, China; 1:500 dilution), or rabbit polyclonal anti-beta-actin antibody (Bioss, Beijing, China; 1:1,500 dilution) and the secondary antibody goat anti-rabbit IgG conjugated with HRP (Bioss, Beijing, China; 1:5,000 dilution).

Techniques: Expressing, Western Blot, Immunofluorescence, Staining